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8:45
YouTube
Nikolay's Genetics Lessons
How to calculate how many times restriction endonuclease will cut DNA
A restriction enzyme, restriction endonuclease, or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes.
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Restriction enzyme DNA Cutting
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14K views · 446 reactions | EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio | Facebook
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