This protocol outlines the basic principles of PCR, provides a methodology that will result in amplification of most target sequences, and presents strategies for optimizing a reaction.

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Protocols that do not use harsh and dangerous chemicals, e.g., phenol, are available online. They produce sufficient quality to enable most routine PCR applications.

View a protocol to perform PCR using Taq DNA Polymerase including materials, reaction setup, and thermocycling conditions for 25 µl and 50 µl reaction sizes.

The protocol in brief You will perform a PCR reaction on you DNA sample to generate multiple copies of a portion of the 16S rRNA gene. The first step involves setting up a master mix containing enough of …

See our reference page on how to design primers for detailed instructions and check out our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes.

Abstract This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation …

Use a fine black Sharpie to label six 0.2 ml PCR tubes with the same ID you labeled your DNA Determine how many PCR reactions, and you will make a “Master mix” that will be used for all the …

May 5, 2025 · If the appropriate protocol does not exist, create a new protocol using the PCR template. Input the name of the protocol, times and temperatures indicated above for each step.

This article provides a detailed summary of a standard PCR protocol, guiding you through the essential steps, critical components, and considerations for successful DNA amplification.